Biomineralisation is the process by which microorganisms mediate and catalyse inorganic reactions so as to form new mineral assemblages. The goal of biomineralisation as a remediation tool is to control microbial processes and their environment so as to sequester and immobilise specific toxic metals within exceptionally strong bonds of neo-formed mineral assemblages. In this way, the bound metals are no longer mobile within a geological time-scale. Applied biomineralisation uses microorganisms, which are isolated from a metal waste environment and are selected for their ability to rapidly catalyse neo-mineral development from dissolved or leachable metals. A typical waste environment will contain microbes that contribute to the mobilisation of metal contaminants as well as microbes that possess the potential for remineralising available metals. Selection and augmenting the correct combination of bacteria and eliminating the competing mineral degrading reactions are the key to applying biomineralisation as an in situ remediation process. Applied biomineralisation harnesses the microbes' ability to stabilise toxic soluble metals into non-mobile mineral assemblages._x000D_
This paper will discuss and demonstrate the biomineralisation process through several case studies including the Summitville mine site in Colorado, the McCoy-Cove mine in Nevada, the Argonaut mine in California, and an historic lead site in Crooksville, Ohio.