Novel microbial isolates that readily oxidise iron and sulfur were recovered from samples collected from a spent chalcopyrite/pyrite test heap. Methods were developed to enrich and isolate microbes that thrive in low pH environments and utilise iron and/or sulfur as sources of energy. Screening of these isolates revealed diverse substrate utilization (sulfur, ferrous ion, chalcopyrite, pyrite, glucose) as well as growth at temperatures between 30 - 50C._x000D_
When native isolates were employed, shake flask leaching tests of chalcopyrite concentrate resulted in higher copper yields in comparison to laboratory reference strains Acidithiobacillus ferrooxidans (DSM 583) and Sulfobacillus thermosulfidooxidans (DSM 9293). After 700 hours several native isolates had leached between 20 - 30 per cent more copper than laboratory reference strains during experiments conducted at 45C._x000D_
This research has established a unique culture collection of mining microbes that readily adapt to Australian conditions. Exploitation of these microbes may result in improved leaching kinetics and copper recovery from Australian chalcopyrite ores as demonstrated from shake flask leaching tests. Whilst this research provides a capability for Australian mineral sulfide ores, this technology can be easily adapted to suit ores of any origin resulting in more effective and efficient microbial leaching.