Much of the research regarding the use of bacteria for the oxidation of sulfide minerals has been conducted using pure bacterial strains or synthetic minerals, often under ideal conditions, which does not reflect the conditions commonly encountered in commercially operating plants. This has also led to much conjecture as to the exact mechanism by which bacteria are able to enhance sulfide oxidation and as to which species of bacterial microflora are the most dominant. Recent advances in 16S rNA sequencing has confirmed that many of the bacterial species once thought to dominate commercial oxidation circuits were simply more willing to grow under the culturing conditions used and may only play a minor role in the oxidation process (Rawlings, 2002). Consequently this research will look at bacterial oxidation under conditions more reflective of commercial operations, focusing on the presence of organic carbonaceous material within a refractory sulfide orebody to be sourced from the Fosterville deposit of Perseverance Corporation Limited.