A novel approach to validate size distributions obtained from bubble sizing techniques is introduced. The experimental rationale consists in verifying whether the output features the anticipated bi-modality when two uni-modal populations (ca. 0.5 and 5 mm) are mixed in a bubble column. The degree of deviation is quantified by comparing the calculated and real proportions on a volumetric basis. A sampling-followed-by-imaging technique was used to proof the concept. The results showed the anticipated bi-modality in which the real and calculated proportions corresponded with a deviation of less than 15 per cent, considered good given the severity of the test. However, the deviation was correlated to the superficial gas velocity (Jg) in the column and was consistently against smaller bubbles. Possible sources of bias are suggested.