Three polymers of varying chemistries and molecular weights have been used to depress talc in a model sulfide ore consisting of talc, pentlandite, and chalcopyrite. Flotation testing revealed a distinct hierarchy of effectiveness of the polymers in the depression of talc (HP Starch > Polymer-H > Dextrin WY) but a different order of effectiveness for depression selectivity (Dextrin WY > Polymer-H > HP Starch). Adsorption isotherm determinations for the gangue and valuable mineral phases indicate that the differences in selectivity are partially due to adsorbed amount variations, with Dextrin WY adsorbing marginally less on the sulfides than Polymer-H. Contact angle measurements on packed particle beds have revealed that the polymers have differing abilities to reduce the contact angle of talc and the sulfide minerals. An explanation of the observed trends is given in terms of the binding mechanisms of the polymers on the talc and the sulfide mineral phases. Order of addition effects between collector and depressant were also investigated using adsorption isotherm determinations for the polymers in the presence of a pre-adsorbed layer of collector and vice versa. Adsorption of the polymers was not affected by the presence of the collector. Similar results were obtained for the adsorption of collector in the presence of pre-adsorbed layers of polymer.