A study of the bacterial oxidation of naturally occurring high-purity sulphide minerals (FeS2, Fel_xS, CuFeS2, Cu2S) has been conducted using a mesophilic strain of T. 6ejtAooLcdcth4. As well as metal analyses and solution pH, partitioning of sulphide sulphur into elemental sulphur and sulphate ion has been monitored. Partially oxidised sulphide substrates have been characterised at regular time intervals by X-ray diffraction methods and extra-cellular organic compounds reporting into leach solution have been identified by'thin layer chromatography. The results indicate that the S/SO4 2 partitioning of the sulphide substrates is independent of cell inoculum size and initial pulp density and varies with solution pH, Eh, leaching time and mineralogical properties of the individual sulphide minerals. The signifi- cance of results for applications in selective flotation, coal desulphurisation and agglomera- tion has been outlined.