A risk assessment was undertaken of the aquatic ecosystem and bioaccumulation of metals and metalloids in fish at the Leichhardt River, Mount Isa, Queensland. Residual contamination of metals and metalloids in Leichhardt River sediment was assessed and arose from mine practices using river discharge in the 1940s. The ecological risk assessment of sediments involved comparison of the concentrations of heavy metals and arsenic with those of the ANZECC/ARMCANZ (2000) Interim Sediment Quality Guidelines (ISQG). Results indicated that all sites including the background site exceeded one or both the ISQG-high and ISQG-low trigger values based on total concentrations. A lesser number of sites exceeded the ISQG-low trigger values following the 1 M hydrochloric acid extract step, which was used as an indication of bioavailability. An additional risk from particulate matter in river sediment is that acute human toxicity from the consumption of fish contaminated with metals and metalloids is unlikely. The potential risk was assessed by comparing with the maximum levels set by FSANZ (2010) for fish consumption by children and adults. In general, the liver of the fish have higher heavy metal and metalloid concentrations compared to the muscle. Frequent or regular consumption of fish from the Leichhardt River that exceed maximum levels (ML) of heavy metals and metalloids is not recommended. Risk analysis showed that an adult can consume up to 5 kg of fish fillets every day from fish caught at the man-made Lake Moondarra downstream on Leichhardt River and still be within safe consumption levels whilst a child can eat up to 1 kg daily.CITATION:Noller, B N, Ng, J C, Matanitobua, V, Harris, H H, Zheng, J and Huynh, T, 2014. Assessment of ecotoxicology and health risk from bioaccumulation in fish of heavy metals and metalloids from historical mine practices in the Leichhardt River, Queensland, Australia, in Proceedings Life-of-Mine 2014 , pp 337-348 (The Australasian Institute of Mining and Metallurgy: Melbourne).