Cyanide has been used for over a century worldwide for gold extraction. However, the presence of several metals and metalloids in ore requires greater amount of cyanide for an efficient gold recovery due to their solubilisation in conjunction with gold. The extraction residues are discharged into tailing storage facilities (TSFs) and may cause bird mortality if there is exposure to cyanide-bearing wastewater exceeding the toxic threshold. The toxicity arises from free cyanide released from weak acid dissociable (WAD) cyanide complexes. The International Cyanide Management Code (ICMC) has deemed 50 mg/L of WAD cyanide released into TSFs to be safe for wildlife protection. However, this concentration is based on observations of an apparent lack of toxicity rather than a toxicological approach. The National Industrial Chemicals Notification and Assessment Scheme (NICNAS) identified in 2010 that there is a lack of toxicity data to properly assess the environmental significance of cyanide levels at gold mines. Lack of knowledge for the toxicity of free cyanide and metal cyanide complexes to wildlife led to consider alternative direct assessment techniques for designating safe levels. Whilst toxicity may be assessed by using young chickens with animal ethics approval, the traditional bioassay approach to assess cyanide toxicity is impractical for gold mine TSFs. Bioassays using algal species such as Euglena gracilis, a unicellular alga, are widely used for ecotoxicological studies. A unique feature is that the Euglena gracilis Z cell has plant-like while SMZ cell has animal-like characteristics. The aims of this project are to evaluate the suitability of Euglena gracilis Z and SMZ strains to assess the toxicity of free cyanide and both strong (cobalt) and weak (zinc, copper and nickel) acid dissociable metal-cyanide complexes that may be present in TSFs.CITATION:Noller, B N, Ng, J C, Shini, S, Breuer, P L, Sichani-Teimouri, H and Alsaadi, R N, 2014. Development of a toxicity approach to evaluate free cyanide and metal cyanide complexes in waters associated with gold and other mining, in Proceedings Life-of-Mine 2014 , pp 573-590 (The Australasian Institute of Mining and Metallurgy: Melbourne).