Disposal of mine water is a worldwide problem occurring in the coal and gold as well in the metal ore mining areas. Since southern Africa is a (semi) arid region, mining effluents should be treated to qualities such that they can be used for agricultural purposes or that they can be discharged in river system. Studies have shown that partly treated acid mine water can be used for irrigation. A copper mine in Botswana plans to use 5 M/d of treated mine effluent for the irrigation of agricultural product. The results of this study showed that when applying the biological sulfate removal technology the produced sulfide precipitated the metals in the mine effluent, such as nickel, Zn and Mn, to metal sulfide (MeS).