The role of biological agents in the gold mining industry is currently limited to the use of microorganisms in bioleaching and bioremediation. However, there is a variety of possibilities in which biotechnology could aid the mining industry in the future. In this article we discuss the potential for the development of these novel biotechnologies and the role they can play in the exploration for new gold deposits, the processing of ores and the post-mining remediation of sites. The development of these biotechnologies has been enabled by rapid advances in our molecular-level understanding of the role microorganisms play in the solubilisation, dispersion and precipitation of gold. An understanding of the distribution of microbial species in soils overlying mineralisation can be utilised to develop bioindicator systems that assist with gold exploration. An in-depth knowledge of how microorganisms interact with gold complexes is being used to develop in-field biosensors, supporting exploration. Processing technologies may be improved based on advances in our understanding of the interactions between microorganisms, ore minerals, cyanide and gold. For instance, cyanide-producing microorganisms are being investigated for use in in situ leaching of gold. In turn, the use of cyanide-utilising microorganisms for the degradation of cyanide is being explored. Combined, the implementation of these biotechnologies in the gold mining sector may revolutionise the industry, leading to greener, more efficient exploration for and extraction of gold.CITATION:Zammit, C M, Cook, N, Ciobanu, C L, Brugger, J and Reith, F, 2013. Possible future applications of biotechnologies in the gold industry - a prospective approach, in Proceedings World Gold 2013 , pp 233-244 (The Australasian Institute of Mining and Metallurgy: Melbourne).