Thiourea has been recognised for a long time as an acid-stable complexant for gold. Generally, the use of thiourea has been shown to exhibit enhanced leaching kinetics compared to cyanide but suffers the disadvantage of excessive reagent consumption. Thiourea leaching is particularly com- patible with the extraction of gold from refractory ores. This is due to the need for an oxidative pretreatment to liberate the gold. Such pretreatments typically result in the production of an acidic residue. The application of novel thiourea leaching technologies to the extraction of gold from a bioleached pyrite concentrate was investigated. The interac- tion of leach variables with thiourea consumption and gold extraction was established. An economic assessment of thiourea leaching was compared with conventional cyanidation. The results indicated that gold leaching of the bioleached residue using thiourea compared very favourably with cyanidation.